Application | Comment | Organism |
---|---|---|
drug development | PseB is a potential target for the development of antibiotics | Campylobacter jejuni |
Cloned (Comment) | Organism |
---|---|
gene pseB, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Campylobacter jejuni |
Protein Variants | Comment | Organism |
---|---|---|
D126N | site-directed mutagenesis, the mutant shows about 100fold lower activity with UDP-GlcNAc and with UDP-6-deoxy-6-fluoro-GlcNAc for HF elimination compared to the wild-type enzyme. Upon addition of UDP-4-keto-6-deoxy-GlcNAc to D126N the tightly bound NADPH is immediately oxidized | Campylobacter jejuni |
K127A | site-directed mutagenesis, the mutant shows about 100fold lower activity with UDP-GlcNAc and with UDP-6-deoxy-6-fluoro-GlcNAc for HF elimination compared to the wild-type enzyme. Upon addition of UDP-4-keto-6-deoxy-GlcNAc to K127A the tightly bound NADPH is immediately oxidized | Campylobacter jejuni |
Y135F | site-directed mutagenesis, the mutant shows about 100fold lower activity with UDP-GlcNAc and with UDP-6-deoxy-6-fluoro-GlcNAc for HF elimination compared to the wild-type enzyme, slow oxidation of NADPH upon addition of UDP-4-keto-6-deoxy-GlcNAc to Y135F | Campylobacter jejuni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-GlcNAc | Campylobacter jejuni | the enzyme is involved in the pseudaminic acid biosynthesis, it is responsible for the biosynthesis of 6-deoxyhexose | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hexos-4-ulose + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Campylobacter jejuni | - |
gene pseB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) | Campylobacter jejuni |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hex-4-ulose + H2O | Asp126, Lys127, and Tyr135 are the active site residues | Campylobacter jejuni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Campylobacter jejuni |
0.000037 | - |
wild-type enzyme activity with UDP-6-deoxy-6-fluoro-GlcNAc for elimination of fluoride | Campylobacter jejuni |
0.23 | - |
wild-type enzyme activity with UDP-GlcNAc conversion to UDP-4-keto-6-deoxy-L-IdoNAc | Campylobacter jejuni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-6-deoxy-6-fluoro-GlcNAc | elimination of fluoride from the substrate by the wild-type PseB, no activity by mutant enzymes K127A, D126N, and Y135F | Campylobacter jejuni | UDP-GlcNAc + HF | - |
? | |
UDP-GlcNAc | the enzyme is involved in the pseudaminic acid biosynthesis, it is responsible for the biosynthesis of 6-deoxyhexose | Campylobacter jejuni | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hexos-4-ulose + H2O | - |
? | |
UDP-GlcNAc | the C-5'' epimerization of UDP-4-keto-6-deoxy-L-IdoNAc to UDP-4-keto-6-deoxy-GlcNAc is PseB-catalyzed, and is about 50fold lower than the dehydratase activity | Campylobacter jejuni | UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-hexos-4-ulose + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the short chain dehydrogenase/reductase, SDR, family | Campylobacter jejuni |
PseB | - |
Campylobacter jejuni |
UDP-GlcNAc 5-inverting 4,6-dehydratase | - |
Campylobacter jejuni |
UDP-N-acetylglucosamine 5-inverting 4,6-dehydratase | - |
Campylobacter jejuni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | 30 | assay at | Campylobacter jejuni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Campylobacter jejuni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | dependent on, tightly bound | Campylobacter jejuni |